Piper nigrum (Pwèv-nwè) : medicinal properties in alcoholic extraction

Deng Y et al, Anti-cancer effects of Piper nigrum via inducing multiple molecular signaling in vivo & in vitro. J. Ethnopharmacol. 2016, 188, 87–95-Abstract:  Piper nigrum is widely used as a folk medicine including usage for pain relief, fevers, as well as an anti-cancer agent. However the crude extract of piperine free P. nigrum (PFPE), which inhibits breast cancer, and its mechanisms are still being kept secret. This research aims to elucidate the anti-cancer effects of PFPE & its mechanisms. Materials and methods Anti-cancer effects of PFPE were investigated in N-nitroso-N-methylurea (N…

EE GCL A et al, Pellitorine, a Potential Anti-Cancer Lead Compound against HL60 & MCT-7 Cell Lines & Microbial Transformation of Piperine from Piper Nigrum, Mol, 15(4):2398-2404, 2010-Abstract: Pellitorine (1), which was isolated from the roots of Piper nigrum, showed strong cytotoxic activities against HL60 & MCT-7 cell lines. Microbial transformation of piperine (2) gave a new compound 5-[3,4-(methylenedioxy)phenyl]-pent-2-ene piperidine (3). Two other alkaloids were also found from PN. They are (E)-1-[3',4'-(methylene-dioxy)-]piperidine (4) & 2,4-tetradecadienoic acid isobutyl amide (5).…

Liyanagamage DSN et al, Dual mechanisms of a Sri Lankan traditional in the improvement of pancreatic beta cell functions & restoration of lipoprotein alterations in streptozotocin induced diabetic rats, JL Ethnopharmacol, 267:113613. 2021-Abstract: Traditional polyherbal preparations have been utilized in Sri Lanka since ancient times & have gained a wide acceptance throughout the country. Although an extensive body of evidence supports the use of traditional herbal mixtures in the treatment of diabetes mellitus, only a few polyherbal mixtures have been subjected to systematic scientific in…

Sriwiriyajan S et al, Anticancer & Cancer Prevention Effects of Piperine-Free Piper nigrum Extract on N-nitrosomethylurea-Induced Mammary Tumorigenesis in Rats. Cancer Prev. Res. 2016, 9, 74–82,

Sundaram RL et al, Polyherbal Formulation as a Therapeutic Strategy for Lung Cancer: Phytochemical Profiling, Molecular Docking, & Pharmacological Evaluation, Asian Pac Jl Cancer Prev, 26(5):1841-52.2025-Abstract: Globally, lung cancer continues to be the primary cause of both cancer incidence & death. The disease is multifaceted, influenced by numerous external factors & the individual's genetic or epigenetic predisposition to its onset & timing. Upon diagnosis, fewer than one-third of patients present with localized disease amenable to curative multimodal therapy; the remainder have metas…

Chen SY et al. Piperlongumine suppresses proliferation of human oral squamous cell carcinoma through cell cycle arrest, apoptosis & senescence. Int J Mol Sci.17(4) : 2016-Abstract :oral squamous cell carcinoma (OSCC), an aggressive cancer originating in the oral cavity, is one of the leading causes of cancer deaths in males worldwide. This study investigated the antitumor activity & mechanisms of piperlongumine (PL), a natural compound isolated from Piper longum L., in human OSCC cells. The effects of PL on cell proliferation, the cell cycle, apoptosis, senescence & reactive oxygen species …

BHOSAL SS et al, Therapeutic potential of Bhiramiyadhi bhavanai choornam in ameliorating scopolamine-induced impaired learning & memory in rats: neuroprotective effects & mechanistic insights, Res Jl. Pharm. & Tech, 17(2):553-62. 2024-ABSTRACT: This study Intended to explore Bhiramiyadhi bhâvanâ choornam (BBC) effect on scopolamine-induced Impaired learning & memory in rats. BBC was prepared & subjected to organoleptic & phytochemical investigations. Scopolamine (2mg/kg, i.p.) was injected into all animals except for the vehicle control group. Donepezil 1mg/kg & BBC (215, 430, & 860mg/kg al…

Sriwiriyajan S et al, (−)-Kusunokinin & piperloguminine from Piper nigrum: An alternative option to treat breast cancer. Biomed. Pharmacother. 2017, 92, 732–43-Abstract : Several studies have reported that active compounds isolated from Piper nigrum possess anticancer properties. However, there are no data on anticancer activity of (-)-kusunokinin & piperlonguminine. The purposes of this study were to isolate active compounds from PN & identify the molecular mechanisms underlying growth & apoptosis pathway in breast cancer cells. Two bioactive compounds, (-)-kusunokinin & piperlonguminine, …

WU R et al, piper nigrum inhibits the growth of human colorectal cancer HT-29 cells by inducing p53 mediated apoptosis, Pharmaceuticals, 76 :1325, 2023-Abstract: Colorectal cancer (CRC) is a prevalent malignancy of the digestive tract with the second highest mortality rate globally. Piper nigrum is a widely used traditional medicinal plant, exhibiting antitumor activity against various tumor cells. At present, research on the effect of Piper nigrum on CRC is limited to in vitro cytotoxicity, lacking comprehensive mechanism investigations. This study aimed to explore the inhibitory effect & …

Viet Phong, N et al, Anti-inflammatory activity & cytotoxicity against ovarian cancer cell lines by amide alkaloids & piperic esters isolated from Piper longum fruits: In vitro assessments & molecular docking simulation. Bioorg. Chem. 2022, 128, 106072-Abstract : Three new amide alkaloids, piperlongumamides D–F (14, 19, & 32); a new piperic ester, piperlongumester A (45); & two new natural compounds, methyl (2E, 4Z)-5-(1, 3-benzodioxol-5-yl) penta-2, 4-dienoate (46) & trans-piperolein B ester (47), along with 41 known compounds were isolated from the fruits of Piper longum L. Their structur…

Ginzburg S et al, Piperlongumine inhibits NF-kappaB activity & attenuates aggressive growth characteristics of prostate cancer cells. Prostate 2014;74:177-86.-Abstract: Elevated NF-κB activity has been previously demonstrated in prostate cancer cell lines as hormone-independent or metastatic characteristics develop. We look at the effects of piperlongumine (PL), a biologically active alkaloid/amide present in piper longum plant, on the NF-κB pathway in androgen-independent prostate cancer cells. Methods: NF-κB activity was evaluated using Luciferase reporter assays and Western blot analysis…

Rout J et al, In silico investigation of spice molecules as potent inhibitor of SARS-CoV-2. Chemrxiv, 2020-Abstract: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a novel infectious disease that is in rapid growth. Several trials are going on worldwide to find a solution for this pandemic. The viral replication can be blocked by inhibiting the receptor-binding domain (RBD) of SARS-CoV-2 spike protein (SARS-CoV-2 RBD Spro) & the SARS-CoV-2 main protease (SARS-CoV-2 Mpro). The binding of potential small molecules to these proteins can inhibit the replication & transcript…

HO X et al,. Identification of Piperlongumine as Potent Inhibitor of Necroptosis, Des Devel Ther, :1387-94.2023-Abstract: Excessive necroptosis contributes to the pathogenesis of several inflammatory & neurodegenerative diseases. Here, using a high-throughput screening approach, we investigated the anti-necroptosis effects of piperlongumine, an alkaloid isolated from the long pepper plant, in vitro & in a mouse model of systemic inflammatory response syndrome (SIRS). Methods: A natural compound library was screened for anti-necroptosis effects in cellular. The underlying mechanism of actio…

Liyanagamage DSNK et al, Acute & Subchronic Toxicity Profile of a Polyherbal Drug Used in Sri Lankan Traditional Medicine, Evid Baser Comp & Alter Med, 2189189, 2020-Abstract: A polyherbal drug composed of leaves of Murraya koenigii L. Spreng, cloves of Allium sativum L., fruits of Garcinia quaesita Pierre, & seeds of Piper nigrum L. is a popular drug which has been used by indigenous practitioners in Sri Lanka for the treatment of diabetes mellitus & dyslipidemia. The acute toxicity assessment was conducted, following a single oral dose of 0.25-2.0 g/kg in healthy rats, & rats were observe…

Gupta S et al, Evaluation of in vitro aldose reductase inhibitory potential of alkaloidal fractions of Piper nigrum, Murraya koenigii, Argemone mexicana, & Nelumbo nucifera,  Asian Pac Jl of Trop Biomed, 25(2):1-11, 2013-Abstract: Aldose reductase is primarily involved in development of long-term diabetic complications due to increased polyol pathway activity. The synthetic aldose reductase inhibitors are not very successful clinically. Therefore, the natural sources may be exploited for safer & effective aldose reductase inhibitors. Methods: In the present study, the aldose reductase inhib…

Vijayakumar RS et al, Efficacy of piperine, an alkaloidal constituent from Piper nigrum on erythrocyte antioxidant status in high fat diet & antithyroid drug induced hyperlipidemic rats, Cell Biochem Funct, 24(6):491-8, 2006-Abstract: The main aim of this study was to investigate the effect of piperine on erythrocyte antioxidant status in high fat diet (HFD) & antithyroid drug induced hyperlipidemic rats. Male Wistar rats were divided into eight groups. The first four groups were fed a control diet & in addition were given respectively 1% carboxymethyl cellulose (CMC); 10 mg/kg body weight…

Awasthee N et al, Piperlongumine, a piper alkaloid, enhances the efficacy of doxorubicin in breast cancer: involvement of glucose import, ROS, NF-κB & lncRNAs, Apoptosis, 27(3-4):261-82. 2022-Abstract: Piperlongumine (PL, piplartine) is an alkaloid derived from the Piper longum L. (long pepper) roots. Originally discovered in 1961, the biological activities of this molecule against some cancer types was reported during the last decade. Whether PL can synergize with doxorubicin & the underlying mechanism in breast cancer remains elusive. Herein, we report the activities of PL in numerous bre…