Zingiber zerumbet (Jenjanb wouj) : vertus médicinales en extraction alcoolique

Malek SNA et al, Essential oil of Zingiber ottensii Valet and Zingiber zerumbet

Albaayit SFA et al, Zerumbone Induces Growth Inhibition of Burkitt’s Lymphoma Cell Line Via Apoptosis. Nat. Volatiles Essent. Oils. 8(3): 56-63. 2021-Abstract: Zerumbone (ZER), a natural compound has been extracted from Zingiber zerumbet with known pharmacological activities. The aim was to determine the anti-human Burkitt’s lymphoma (Raji) cell effect of ZER. The 3-(4,5-dimethylthiazol-2-yl)-2,5, - diphenyltetrazolium bromide (MTT) assay was used to determine cytotoxic effect while the Annexin-V-fluorescein isothiocyanate/propidium iodide-PI flow cytometric assays were used to determine ap…

Khalid MH et al, Antinociceptive Effect of the Essential Oil of Zingiber zerumbet in Mice: Possible Mechanisms. J. Ethnopharmacol. 137(1): 345-51. 2011-Abstract: The present study was conducted to investigate the possible mechanism of actions underlying the systemic antinociception activity of the essential oil of Zingiber zerumbet (EOZZ) in chemical-induced nociception tests in mice. Materials & methods: Acetic acid-induced abdominal constriction, capsaicin-, glutamate- a&phorbol 12-myristate 13-acetate-induced paw licking tests in mice were employed in the study. In all experiments, EOZZ …

Kader G et al, antimicrobial activities of the rhizome extract of Zingiber zerumbet Linn, Asian Pacific J of Trop Biomed, 1(5):409-12, 2011-Abstract: To investigate antimicrobial effects of ethanolic extract of Zingiber zerumbet & its chloroform & petroleum ether soluble fractions against pathogenic bacteria & fungi. Methods: The fresh rhizomes of ZZ were extracted in cold with ethanol (4.0 L) after concentration. The crude ethanol extract was fractionated by petroleum ether & chloroform to form a suspension of ethanol extract (15.0 g), petroleum ether fraction (6.6 g) & chloroform soluble …

Chang CJ et al, Regulation of lipid disorders by ethanol extracts from Zingiber zerumbet in high-fat diet-induced rats, Food Chem, 132(1):460-7, 2012-Abstract: The aim of this study was to investigate the antihyperlipidaemic effects of the ethanol extract of Zingiber zerumbet (L) Smith (EEZZ). After being fed a high-fat diet (HFD) for 2 weeks, rats were dosed orally with EEZZ (100, 200 or 300 mg/kg) or fenofibrate (100 mg/kg) once daily for 8 weeks. EEZZ (300 mg/kg/day) produced effects similar to fenofibrate in reducing body weight gain, visceral fat-pad weights & plasma lipid levels. EEZZ…

Rahman HS et al, Zerumbone Induces G2/M Cell Cycle Arrest & Apoptosis Via Mitochondrial Pathway in Jurkat Cell Line. Nat. Prod. Commun. 9(9): 1237-42. 2014-Abstract: This investigation determined the anticancer properties of zerumbone (ZER) on the human T-cell (Jurkat) line using the MTT assay, microscopic evaluations, flow cytometric analyses, & caspase activity estimations. The results showed that ZER is selectively cytotoxic to Jurkat cells in a dose & time-dependent manner with IC50 of 11.9 ± 0.2, 8.6 ± 0.5 & 5.4 ± 0.4 μg/mL at 24, 48 & 72 hours of treatment, respectively. ZER did not p…

Albaayit SFA et al, Enzyme Inhibitory Properties of Zerumbone. Pakistan J. Agric. Sci. 58(3): 1207-9. 2021-

Koga AY et al, Several aspects of Zingiber zerumbet: a review, Rev Bras de Farmacogn, 26(3):385-91, 2016-Abstract:Zingiber zerumbet is a perennial, aromatic & tuberose plant that grows in humid locations. Also known as bitter ginger, ZZ is traditionally found throughout Asia, where it is widely used in foods, beverages & for ornamental purposes. The viscous juice present in the inflorescence of the plant is rich in surfactants & is also known as “ginger shampoo”. The rhizome can be macerated in ethanol & used as a tonic & a stimulant. In Brazil ZZ is found in the Amazon region, in rural are…

Chang CJ et al, Absence of Genotoxic & Mutagenic Effects of Zingiber zerumbet (L.) Smith (Zingiberaceae) Extract. Evidence-based Comp Alter Med. 2012-

Koga AY et al, Several Aspects of Zingiber zerumbet: A Review. Rev. Bras. Farmacogn. 26(3): 385-91. Doi: 10.1016/j.bjp.2016.01.006. 2016-

Malek SNA et al. Essential Oils of Zingiber ottensii Valet. & Zingiber zerumbet (L.) Sm. from Sabah,

Khalid MH et al, Antinociceptive effect of the essential oil of Zingiber zerumbet in mice: Possible mechanisms, J of Ethnopharmacol, 137(1-:345-51, 2011-Abstract: The present study was conducted to investigate the possible mechanism of actions underlying the systemic antinociception activity of the essential oil of Zingiber zerumbet (EOZZ) in chemical-induced nociception tests in mice. Materials & methods: Acetic acid-induced abdominal constriction, capsaicin-, glutamate- & phorbol 12-myristate 13-acetate-induced paw licking tests in mice were employed in the study. In all experiments, EOZZ…

Kader G et al, Antimicrobial Activities of the Rhizome Extract of Zingiber zerumbet Linn. Asian Pac. J. Trop. Biomed. 1(5): 409-12. 2011-

Rahman HS et al. Biomedical Properties of Natural Dietary Plant Metabolite, Zerumbone, in Cancer Therapy & Chemoprevention Trials. Biomed Res. Int. 1-20. Doi: 10.1155/2014/920742. 2014-

Sutardi LN et al, Indonesian Wild Ginger (Zingiber sp) Extract: Antibacterial Activity against Mycoplasma gallisepticum. IOSR J. Pharm. 5(10): 59-64. 2015-

Yu F et al. Isolation & functional Characterization of a β-eudesmol Synthase, a New Sesquiterpene Synthase from Zingiber zerumbet Smith. FEBS Lett. 582(5): 565-72. 2008-Abstract: In this paper, we have identified a new sesquiterpene synthase gene (ZSS2) from Zingiber zerumbet Smith. Functional expression of ZSS2 in Escherichia coli & in vitro enzyme assay showed that the encoded enzyme catalyzed the formation of β-eudesmol & five additional by-products. Quantitative RT-PCR analysis revealed that ZSS2 transcript accumulation in rhizomes has strong seasonal variations. To further confirm the …

Chia JSM et al, Zerumbone Ameliorates Neuropathic Pain Symptoms via Cannabinoid & PPAR Receptors Using In Vivo and In Silico Models, Molecules, 26(13):3849, 2021-Abstract: Neuropathic pain is a chronic pain condition persisting past the presence of any noxious stimulus or inflammation. Zerumbone, of the Zingiber zerumbet ginger plant, has exhibited anti-allodynic & antihyperalgesic effects in a neuropathic pain animal model, amongst other pharmacological properties. This study was conducted to further elucidate the mechanisms underlying zerumbone's antineuropathic actions. Research on thera…

Singh, B et al Chemical Composition & Biological Activity of the Essential Oil of Rhizome of Zingiber zerumbet (L.) Smith. J. Pharmacogn. Phytochem. 3(3): 130-133. 2014-Abstract: The aim was designed to study the biological activity & chemical composition of essential oil of Zingiber zerumbet. The essential oil extracted from the rhizome of the plant was analysed by gas chromatography-mass spectroscopy & its major components amounting to 98.4 % were found to be zerumbone (75.2%), a- caryophyllene (7.1%), camphene (5.1%), eucalyptol (2.4%), & camphor (3.0%). Antioxidant activity & total phen…

Jantan I et al, Platelet-activating factor (PAF) receptor-binding antagonist activity of Malaysian medicinal plants. PubMed, 2005-Abstract: Forty-nine methanol extracts of 37 species of Malaysian medicinal plants were investigated for their inhibitory effects on platelet-activating factor (PAF) binding to rabbit platelets, using 3H-PAF as a ligand. Among them, the extracts of six Zingiberaceae species (Alpinia galanga Swartz., Boesenbergia Pandurata Roxb., Curcuma Ophiorrhiza Val., C. aeruginosa Roxb., Zingiber officinale Rosc. & Z. zerumbet Koenig.), two Cinnamomum species (C. altissimum K…

Jani NHM et al. The effect of Zingiber zerumbet rhizome ethanolic extract on sexual behaviours & sperm parameters in high fat diet-induced obese rats. J Applied Pharm Sci, 2021; 11(05):079–84-Abstract: Obesity is a worldwide health problem that may impose infertility risk in males. This study was conducted to determine the effect of Zingiber zerumbet rhizome ethanolic extract (ZZREE) on sexual behaviours & sperm parameters in diet-induced obese rats. Male Sprague Dawley rats were fed with high fat diet (HFD), ad libitum daily for 6 weeks to induce obesity. Twenty obese rats were randomly di…

Wahab IMA et al, Subcritical Water Extraction (SWE) of Zingiber zerumbet using Two Level Full Factorial Design. Malaysian J. Fundam. Appl. Sci. 15(2): 139-45. 2019-

Chane-Ming J et al, Chemical Composition of the Essential Oil from Rhizomes, Leaves & Flowers of Zingiber Zerumbet Smith from Reunion Island. J. Essent. Oil Res. 15(3): 202-5. 2003-Abstract: sixty-nine constituents were identified in essential oil from the rhizomes, leaves & flowers of Zingiber zerumbet Smith from Reunion Island. The oils were obtained by steam distillation. The oils obtained from rhizomes were rich in zerumbone (37%), α-humulene (14.4%) & camphene (13.8%). The oils from leaves and flowers differed appreciably from that from rhizomes by the presence of large amounts of (E)-…

Wu Y et al. Contact & Repellant Activities of Zerumbone & Its Analogues from the Essential Oil of Zingiber zerumbet (L.) Smith against Lasioderma serricorne. J. Oleo Sci. 66(4): 399-405, 2017-Abstract: The contact toxicity and repellent activities of the essential oil extracted from the rhizomes of Zingiber zerumbet (L.) Smith (Zingiberaceae) was evaluated against cigarette beetles (Lasioderma serricorne). The essential oil obtained by hydrodistillation was investigated by GC-FID & GC-MS. The main constituents of the essential oil were zerumbone (40.2%), α-caryophyllene (8.6%), humulene epo…

Dash B et al. Zingiber zerumbet Rhizome Essential Oil Induces Cytotoxicity, Apoptosis & Cell Cycle Arrest in Jurkat Cells. J. Essent. Oil-Bearing Plants. 25(3): 639-60. 2022-Abstract: Zingiber zerumbet (L.) Roscoe ex Sm. is an important medicinal herb from the Zingiberaceae family that has long been used as a folk medicine to treat a variety of diseases. In the present study, rhizome essential oil of ZZ (ZZREO) was examined through Gas Chromatography-Mass Spectroscopy (GC/MS). Total 28 compounds were identified accounting 91.83% of the total oil. Zerumbone (71.93 ± 0.43%) was identified to …

Khera et al, Zerumbone: A Magical Phytochemical. Int. J. Heal. Sci. Res: 73-79. https://www.ijhsr.org/IJHSR_Vol.10_Issue:2020/10.pdf.

Malek SNA et al, Essential oil of Zingiber ottensii Valet and Zingiber zerumbet

Dung NX et al, The constituents of the rhizome oil of Zingiber zerumbet (L.) Sm. from Vietnam. J Essent Oil Res. 1993; 5: 553-55.

Haque M et al, Standardized extract of Zingiber zerumbet suppresses LPS-induced proinflammatory responses through NF-κB, MAPK & PI3K-Akt signaling pathways in U937macrophages, Phytomed, 54:195-205, 2019-Abstract: Zingiber zerumbet rhizome has been used as spices & in traditional medicine to heal various immune-inflammatory related ailments. Although the plant was reported to have potent anti-inflammatory & immunosuppressive properties by several studies, the molecular mechanisms underlying the effects have not been well justified. Purpose: The study was carried out to investigate the molecu…

Murakami A et al, Identification of Zerumbone in Zingiber zerumbet Smith as a Potent Inhibitor of 12- O -Tetradecanoylphorbol-13-acetate-induced Epstein-Barr Virus Activation. Biosci. Biotechnol. Biochem. 63(10): 1811-2. 1999-

Ajish KR et al, Studies on α-glucosidase, aldose reductase & glycation inhibitory properties of sesquiterpenes & flavonoids of Zingiber zerumbet Smith, Nat Prod Res, 29:10, 2015-Abstract: Eight known phytochemicals, four sesquiterpenes & four flavonoids of Zingiber zerumbet were screened against α-glucosidase enzyme, aldose reductase enzyme & antiglycation property under in vitro conditions. The results established kaempferol-3-O-methylether as a potent inhibitor of α-glucosidase enzyme with an IC50 value of 7.88 μM. In aldose reductase enzyme inhibition assay, all the compounds except zeru…

Varier NS et al. Chemical Examination of the Rhizomes of Zingiber zerumbet, Smith. Proc. Indian Acad. Sci. - Sect. A. 20(5): 5257-60. Doi: 10.1007/BF03046420. 1943-

Shieh YH et al, Zerumbone Enhances the Th1 Response & Ameliorates Ovalbumin-induced Th2 Responses & Airway Inflammation in Mice. Int. Immunopharmacol. 24(2): 383-91. 2015--Abstract: Zerumbone is a sesquiterpene compound isolated from the rhizome of wild ginger, Zingiber zerumbet Smith. The rhizomes of the plant are used as a spice & traditional medicine. Zerumbone was shown to possess anticarcinogenic, anti-inflammatory, & antioxidant properties. However, the antiallergic activity & the underlying mechanism of zerumbone have not been reported. Herein, we investigated the immunomodulatory ef…

Akhtar NMY et al, Standardized Ethanol Extract, Essential Oil & Zerumbone of Zingiber Zerumbet Rhizome Suppress Phagocytic Activity of Human Neutrophils. BMC Comp Alter Med. 19(1): 331, 2019 Abstract:  Zingiber zerumbet rhizome & its bioactive metabolites have previously been reported to exhibit innumerable pharmacological properties particularly anti-inflammatory activities. In the present study, the 80% ethanol extract, essential oil & Zerumbone of ZZ rhizomes were explored for their in vitro immunosuppressive properties on chemotaxis, CD11b/CD18 expression, phagocytosis & chemiluminescen…

Albaayit SFA et al, Zerumbone Induces Growth Inhibition of Burkitt’s Lymphoma Cell Line Via Apoptosis. Nat. Volatiles Essent. Oils. 8(3): 56-63. 2021-Abstract: Zerumbone (ZER), a natural compound has been extracted from Zingiber zerumbet with known pharmacological activities. The aim was to determine the anti-human Burkitt’s lymphoma (Raji) cell effect of ZER. The 3-(4,5-dimethylthiazol-2-yl)-2,5, - diphenyltetrazolium bromide (MTT) assay was used to determine cytotoxic effect while the Annexin-V-fluorescein isothiocyanate/propidium iodide-PI flow cytometric assays were used to determine ap…

Sithara T et al. Zerumbone, a Cyclic Sesquiterpene from Zingiber zerumbet Induces Apoptosis, Cell Cycle Arrest, and Antimigratory Effects in SW480 Colorectal Cancer Cells. J. Agric. Food Chem. 66(3): 602-12. 2018-

Abdelwahab SI et al. Regression of Cervical Intraepithelial Neoplasia by Zerumbone in Female Balb/c Mice Prenatally Exposed to Diethylstilbestrol: Involvement of Mitochondria-regulated Apoptosis. Exp. Toxicol. Pathol. 62(5): 461-9. Doi: 10.1016/j.etp.2009.06.005. 2010-

Baby S et al. High Content of Zerumbone in Volatile Oils of Zingiber zerumbet from Southern India & Malaysia. Flavour Fragr. J. 24(6): 301-8. 2009-Abstract: Zerumbone is a sesquiterpene phytochemical with potential anticancer, anti-inflammatory, anti-HIV & other biological activities, most abundantly found in Zingiber zerumbet (L.) Smith. Previous studies reported 12.6–73.1% of zerumbone in ZZ rhizome oils from various geographical locations. In a careful review of the literature, we found that most of the previous gas chromatographic profiling studies on volatile oils of ZZ were inadequate…

da Silva TM et al, Zerumbone from Zingiber zerumbet (L.) smith: A Potential Prophylactic and Therapeutic Agent against the Cariogenic Bacterium Streptococcus Mutans. BMC Comp & Alter Med. 18(1): 1-9, 2018-Abstract: Essential oil obtained from rhizomes of the Zingiber zerumbet (L.) Smith (popularly known in Brazil as bitter ginger) is mainly constituted by the biomolecule zerumbone, which exhibit untapped antimicrobial potential. The aim of this study was to investigate the antimicrobial activity of the zerumbone from bitter ginger rhizomes against the cariogenic agent Streptococcus mutans. …

Gopalsamy B et al, Zerumbone-Induced Analgesia Modulated via Potassium Channels & Opioid Receptors in Chronic Constriction Injury-Induced Neuropathic Pain, Molecules, 25(17) :3880, 2020-Abstract: Zerumbone, a monocyclic sesquiterpene from the wild ginger plant Zingiber zerumbet (L.) Smith, attenuates allodynia & hyperalgesia. Currently, its mechanisms of action in neuropathic pain conditions remain unclear. This study examines the involvement of potassium channels & opioid receptors in zerumbone-induced analgesia in a chronic constriction injury (CCI) neuropathic pain mice model. Male Insti…

Wahab SIA et al, In Vitro Ultra morphological Assessment of Apoptosis Induced by Zerumbone on (HeLa). J. Biomed. Biotechnol. 1-10. 2009-Abstract:

Wahab SIA et al, Anti-tumor Activities of Analogues Derived from the Bioactive Compound of Zingiber Zerumbet. Int. J. Cancer Res. 4(4): 154-159. 2008-Abstract: The aim of this study is to evaluate some derivatives of Zerumbone for their anti-tumor effects towards human cervical cancer cell lines (HeLa). The MTT tetrazolium salt colorimetric assay was utilized to evaluate the cytotoxic effects of ZER, cisplatin & the derivatives were n-Butylbenzene (compound 5), 1,1`-(4-Chlorobutylidene) bis(4-fluoro-benzene) (compound 6), alpha, alpha-Diphenyl-gamma-butyrolactone (compound 7) & (1,4`-Bipipe…

Jantan I et al, Inhibitory effect of compounds from Zingiberaceae species on human platelet aggregation,  Phytomed, 15(4):306-9, 2008-Abstract: Twelve compounds isolatedfrom Alpinia mutica Roxb., Kaempferia rotunda Linn., Curcuma xanthorhiza Roxb., Curcuma aromatica Valeton & Zingiber zerumbet Smith (Family: Zingiberaceae) & three synthesized derivatives of xanthorrhizol were evaluated for their ability to inhibit arachidonic acid- (AA), collagen- & ADP-induced platelet aggregation in human whole blood. Antiplatelet activity of the compounds was measured in vitro by the Chrono Log whole blo…

Tewtrakul S et al, Anti-allergic Activity of Some Selected Plants in the Zingiberaceae Family. J. Ethnopharmacol. 109(3): 535-8. 2007-Abstract: Ethanolic & water extracts, together with volatile oils from the rhizomes of six selected Zingiberaceous plants, including Curcuma mangga, Kaempferia galanga, Kaempferia parviflora, Zingiber cassumunar, Zingiber officinale & Zingiber zerumbet were investigated for their anti-allergic activities using a RBL-2H3 cell line. The ethanolic (EtOH) extract of K parviflora exhibited the most potent anti-allergic effect against antigen-induced beta-hexosamin…

Ruslay S et al, Characterization of the components present in the active fractions of health gingers (Curcuma xanthorrhiza & Zingiber zerumbet) by HPLC–DAD–ESIMS, Food Chem, 104(3):1183-91, 2007-Abstract: Curcuma xanthorrhiza & Zingiber zerumbet are two of the most commonly used ingredients in Indo-Malaysian traditional medicines, health supplements & tonics. Recently, a number of products derived from the aqueous extracts of these species have appeared in the market in the form of spray-dried powder packed in sachet or bottle. On-line high performance liquid chromatography, coupled with di…

Tzeng TF et al, Zerumbone, a Phytochemical of Subtropical Ginger, Protects against Hyperglycemia-Induced Retinal Damage in Experimental Diabetic Rats. Nutrients. 8(8): 1-14. 2016-Abstract: Diabetic retinopathy (DR), the most ordinary & specific microvascular complication of diabetes, is a disease of the retina. Zerumbone (ZER) is a monocyclic sesquiterpene compound, & based on reports, it is the predominant bioactive compound from the rhizomes of Zingiber zerumbet. The aim of the current study is to evaluate the protective effect of zerumbone against DR in streptozotocin (STZ)-induced diabe…

Azelan NA et al, Antibacterial Activity of Zingiber Officinale & Zingiber Zerumbet Essential Oils Extracted by Using Turbo Extractor Distillatory (TED). J. Teknol. 77(3): 43-47. Doi: 10.11113/jt. v77.6003. 2015-Abstract: Plants Zingiber officinale (ginger) & Zingiber zerumbet (Lempuyang) of Zingiberaceae family have been traditionally used as treatment for stomach problems, nausea, vomiting, epilepsy, sore throat, muscular pains & several other disorders. In this study, essential oils from both plants were investigated for their efficacy on antibacterial activity against two Gram positive (…

Yusmaniar et al, Antibacterial activity of the essensial oils of lempuyang wangi (Zingiber aromaticum val.), lempuyang gajah (Zingiber zerumbet sm.), & Lempuyang emprit (Zingiber amaricans Bl.) on three-gram negative bacteria. Asian J of Applied Sci, 3(2), 290-3.2015-

Bhuiyan MNI et al, Chemical Investigation of the Leaf & Rhizome Essential Oils of Zingiber zerumbet (L.) Smith from Bangladesh. Bangladesh J. Pharmacol. 4(1): 9-12. Doi: 10.3329/bjp. v4i1.845. 2008-Abstract: Zingiber zerumbet (L.) Smith leaf and rhizome oils, obtained by hydrodistillation, were analyzed by gas chromatography mass spectroscopy (GC-MS). Twenty-nine components were identified in the leaf oil. The major components were zerumbone (37.0%); a-caryophyllene (16.4%) & camphene (9.2%). Thirty components were identified in rhizome oil with the main components being in zerumbone (46.8%…

Chien TY et al, Anti-inflammatory Constituents of Zingiber zerumbet. Food Chem. 110(3): 584-9. 2008-Abstract: Zingiber zerumbet Smith has long been used as a botanical medicine for anti-inflammation in Southeast Asia. In this paper, zerumbone (1), 3-O-methyl kaempferol (2), kaempferol-3-O-(2, 4-di-O-acetyl-α-l-rhamnopyranoside) (3), and kaempferol-3-O-(3,4-di-O-acetyl-α-l-rhamnopyranoside) (4) were isolated from the rhizome of ZZ. The inhibitory effects of these compounds on NO & PGE2 production from lipopolysaccharide (LPS)-induced RAW 264.7 macrophages were measured. Among them, 1 and 2 d…

Tzeng T.F et al, the ethanol extract of Zingiber zerumbet attenuates Streptozotocin-induced diabetic nephropathy in rats. Evidence-Based Comp &Alter Med, 2013, 1–8, 2013-Abstract: The ethanol extract from the rhizome of Zingiber zerumbet (EEZZR) has been indicated to possess an insulin-like property by ameliorating hyperglycemia in diabetes. We aimed to investigate whether EEZZR exerts an ameliorative effect on renal damage in diabetes induced by streptozotocin (STZ). Diabetic rats were treated orally with EEZZR (200 & 300 mg kg-1 per day) or metformin (100 mg kg-1 per day) for 8 weeks. The…

Wahab IMA et al. A Comparative Study on Zerumbone Concentration, Radical Scavenging Activity & Total Phenolic Content of Zingiber Zerumbet Extracted via Green & Conventional Extraction. J of Adv Res in Applied Sci & Engin Technol. 1(1): 1-8. 2022-Abstract: Extraction is crucial for herbal extraction to ensure high quality of bioactive compounds from natural herbs. In the current work, Zingiber Zerumbet was extracted via subcritical water extraction (SWE) & is compared with the conventional solvent extraction, Soxhlet. The quality of the extract was investigated in terms of zerumbone concent…

Zakaria ZA et al, Antiinflammatory & Antinociceptive Activities of Zingiber Zerumbet Methanol Extract in Experimental Model Systems. Med. Princ. Pract. 19(4): 287-294. Doi: 10.1159/000312715. 2010—

Huang GC et al, Antitumor Effects of Zerumbone from Zingiber zerumbet in P-388D1 Cells In Vitro & In Vivo. Planta Med. 71(3): 219-24. 2005-Abstract: The fresh rhizome of Zingiber zerumbet (L.) Roscoe ex Smith (Zingiberaceae) is widely used as a folk medicine in Taiwan. In this study, the fresh rhizome was extracted with 95 % EtOH & partitioned with diethyl ether. The antitumor effects of the diethyl ether extract were measured in cultured P-388D (1) cells & in an animal model of P-388D (1)-bearing CDF (1) mice. The results indicated that the extract could induce DNA fragmentation in P-388D …

Aji N et al, Antibacterial Activity & Active Fraction of Zingiber officinale Roscoe, Zingiber montanum (Koenig) Link ex A., & Zingiber zerumbet (L.) Roscoe ex Sm. Against Propionibacterium Acnes. Pharmacogn. J. 14(1): 103-11. 2022-Abstract: Propionibacterium acnes is a commensal bacterium that play a role in developing acne vulgaris on the skin. Antibacterial activity assay in the last decades have shown that Propionibacterium acnes is resistant to several antibiotics. Potential zingiber genera such as Z. officinale, Z. montanum, & Z. zerumbet have potential as antibacterial. Objective: Thi…

Chia JMS et al, Zerumbone alleviates chronic constriction injury-induced allodynia & hyperalgesia through serotonin 5-HT receptors, Biomed Pharmacother, 83:1303-10, 2016-Abstract: Zerumbone, a bioactive sesquiterpene isolated from Zingiber zerumbet (Smith), has shown to exert antiallodynic and antihyperalgesic effects in neuropathic pain mice model in our recent study. The mechanism through which Zerumbone alleviates neuropathic pain has yet to be elucidated. Thus, this study aimed to determine whether the serotonergic system, part of the descending pain modulation pathway, contributes to t…

Abdelwahab SI et al. Zerumbone Induces Apoptosis in T-acute Lymphoblastic Leukemia Cells. Leuk. Res. 35(2): 268-71. 2011-

Khalid MH et al, Antinociceptive Effect of the Essential Oil of Zingiber zerumbet in Mice: Possible Mechanisms. J. Ethnopharmacol. 137(1): 345-51. 2011-Abstract: The present study was conducted to investigate the possible mechanism of actions underlying the systemic antinociception activity of the essential oil of Zingiber zerumbet (EOZZ) in chemical-induced nociception tests in mice. Materials & methods: Acetic acid-induced abdominal constriction, capsaicin-, glutamate- a&phorbol 12-myristate 13-acetate-induced paw licking tests in mice were employed in the study. In all experiments, EOZZ …

Siti NA et al, Modeling & optimization of pilot-scale subcritical water extraction on Zingiber zerumbet by central composite design, IOP Conf, Series: Mat Sci & Engine, 778, 2020-Abstract: Zingiber zerumbet or locally called ‘Lempuyang’ is one of the ginger species extensively cultivated & utilized in the Southeast Asia region. In order to extract valuable ingredients from ZZ, a green & non-toxic extraction process is implemented namely subcritical water extraction (SWE). Modeling & optimization of SWE of ZZ are performed using the central composite design (CCD) by 20 runs with 6 repetition…

Ahmad UK et al, Supercritical Fluid Extraction & Capillary Gas Chromatography of the Rhizomes of Z. zerumbet. J. Microcolumn Sep. 6(1): 27-32. 1994-Abstract: Supercritical carbon dioxide at 60°C & 200 bar has been used to extract the nonpolar components of the dried rhizomes of Zingiber zerumbet. The extract could be fractionated by placing an on-line silica column before the pressure relief valve. The extracts were analyzed using capillary GC & GC-MS. This technique provides an effective means of sample preparation & a relatively fast way of determining the constituents of plant materials.

Kader G et al, antimicrobial activities of the rhizome extract of Zingiber zerumbet Linn, Asian Pacific J of Trop Biomed, 1(5):409-12, 2011-Abstract: To investigate antimicrobial effects of ethanolic extract of Zingiber zerumbet & its chloroform & petroleum ether soluble fractions against pathogenic bacteria & fungi. Methods: The fresh rhizomes of ZZ were extracted in cold with ethanol (4.0 L) after concentration. The crude ethanol extract was fractionated by petroleum ether & chloroform to form a suspension of ethanol extract (15.0 g), petroleum ether fraction (6.6 g) & chloroform soluble …

Ali H et al, Antioxidant, Cytotoxic & Apoptotic Activities of the Rhizome of Zingiber Zerumbet Linn. in Ehrlich ascites Carcinoma Bearing Swiss Albino Mice. Sci. Rep. 1-11. 2022-Abstract: Due to having a long history of traditional uses as a functional food, Zingiber zerumbet was selected here to explore the inherent antioxidant & antineoplastic activities of methanolic extract of its rhizome (MEZZR) against Ehrlich ascites carcinoma (EAC) cells. The rich polyphenol containing MEZZR showed a marked DPPH, ABTS, nitric oxide radicals & lipid peroxidation inhibition activity with an IC50 of 3.…

Chang CJ et al, Regulation of lipid disorders by ethanol extracts from Zingiber zerumbet in high-fat diet-induced rats, Food Chem, 132(1):460-7, 2012-Abstract: The aim of this study was to investigate the antihyperlipidaemic effects of the ethanol extract of Zingiber zerumbet (L) Smith (EEZZ). After being fed a high-fat diet (HFD) for 2 weeks, rats were dosed orally with EEZZ (100, 200 or 300 mg/kg) or fenofibrate (100 mg/kg) once daily for 8 weeks. EEZZ (300 mg/kg/day) produced effects similar to fenofibrate in reducing body weight gain, visceral fat-pad weights & plasma lipid levels. EEZZ…

Sreevani Naga et al, Studies on antioxidant activity of Zingiber zerumbet spent & its constituents through in vitro models. Wudpecker J of Food Technol, 1 (3):48-55, 2013-Abstract: Utilization of Zingiber zerumbet spent, obtained after the extraction of the essential oil, as a bioactive was explored. The dried spent afforded the higher yield (7%) when extracted with aqueous ethanol (60:40) at a material to solvent ratio of 1:20. The total polyphenol content of extracts obtained from extraction of ZZ spent with ethanol, methanol & isopropanol was 11.9, 8.5, 4.9g/100 g gallic acid equivalents…

Rout KK et al, Development & Validation of an HPTLC Method for Analysis of Zerumbone, the Anticancer Marker from Zingiber Zerumbet. Acta Chromatogr. 21(3): 443-52. 2009-Abstract: A simple, accurate, & rapid high-performance thin-layer chromatographic (HPTLC) method for quantification of the anticancer marker zerumbone in different parts of Zingiber zerumbet has been established & validated. Chromatography was performed on aluminium foil-backed silica gel 60 F254 HPTLC plates with the binary mobile phase ethyl acetate–hexane 1.5:8.5. Ultraviolet detection was performed densitometrically at t…

Keong YS et al, Immunomodulatory Effects of Zerumbone Isolated from Roots of Zingiber zerumbet. Pak. J. Pharm. Sci. 23(1): 75-82. 2010- Abstract: In this study, the immunomodulatory effects of Zerumbone isolated from Zingiber zerumbet were investigated by evaluating the effects of this compound towards the lymphocyte’s proliferation (mice thymocytes, mice splenocytes & human peripheral blood mononuclear cells, PBMC), cell cycle progression a cytokine (interleukin 2 & 12) induction. Lymphocyte proliferation assay showed that Zerumbone was able to activate mice thymocytes, splenocytes & PBMC …

Oliveros MB et al, Pharmacognostic studies on Zingiber zerumbet (Linné) Smith & its proposed variety (family Zingiberaceae), Pharmaceut Biol, 20:141–53, 1982-Abstract: The chemistry of the volatile oils & morphology of Zingiber zerumbet (L.) Sm. & a second plant which is being proposed as a variety reveals significant differences between these two plants. Their main physicochemical differences are the following: dried rhizomes of ZZ. yield a volatile oil which is dextrorotatory, congeals at 3°C, & has Zerumbone as its main constituent. Rhizomes of the proposed variety yield an oil which is …

Dae SJ et al, Flavonoids & aromatic compounds from the rhizomes of Zingiber zerumbet, Archives of Pharm Res, 27 (4):386–9, 2004-Abstract:Repeated column chromatography of the CHCI3-soluble fraction of Zingiber zerumbet led to the isolation & identification of two aromatic compounds-hydroxybenzaldehyde (1) & vanillin (2), & six kaempferol derivatives, kaempferol-3,4′,7-O-trimethylether (3), kaempferol-3-O-methylether (4), kaempferol-3,4′-O-dimethylether (5), 4″-O-acetyl afzelin (6), kaempferol-3-O-(4-O-acetyl-α-L-rhamnopyranoside)], 2″,4″-O-diacetyl afzelin (7), kaempferol-3-O-(2,4-O-diacety…

Preshahdin NA et al, Phytochemicals & Antibacterial Activity of Zingiber zerumbet Growing in Negeri Sembilan, Malaysia, Resaerch-gate, 2023-Abstract: the present study is aimed at analyzing the chemical composition of essential oil & screening the phytochemicals in crude extracts from the rhizomes of Zingiber zerumbet (L.) Roscoe ex Sm. (Zingiberaceae) as well as determining their antibacterial activity. The essential oil was extracted from the fresh rhizomes using the hydrodistillation method & analysed by gas chromatography-mass spectrometry (GC-MS). The phytochemicals from the dried rhiz…

Norfazlina MN et al. Cytotoxicity Study of Nigella Sativa & Zingiber zerumbet Extracts, Thymoquinone & Zerumbone Isolated on Human Myeloid Leukemia (HL60) Cell. Open Conf. Proc. J. 4(1): 99-107. 2013-Abstract: The cytotoxicity effects of Nigella sativa (Ns) & Zingiber zerumbet (ZZ) extracts on acute Human myeloid leukemia cell line (HL60) are reported. The HL60 cell line was purchased from the American Tissue Culture Company (ATCC) was treated with petroleum ether (PE) & aqueous (AQ) extracts of Ns & ZZ extracts of ethanol (ET) & Hexane (HEX). In an attempt to explain cytotoxicity effects o…

Huong LT et al. Zingiber zerumbet Rhizome Essential Oil: Chemical Composition, Antimicrobial & Mosquito Larvicidal Activities. European J. Med. Plants. 30(4): 1-12. 2020-Abstract: In the present study, the chemical constituents, antimicrobial & larvicidal potentials of hydrodistilled essential oil from the rhizome of Zingiber zerumbet were evaluated. Study Design: The study was designed in different phases which are: collection of mature rhizomes of ZZ hydrodistillation of essential oils, chemical analysis of the essential oils, determination of the antimicrobial potential & evaluation of l…

Murata K et al, Screening of Spice Extracts Possessing Anti-Acetylcholinesterase Activity & Active Principle of Bitter Ginger, Rhizome of Zingiber Zerumbet. Nat. Prod. Commun. 12(7): 1053-6.2017-Abstract: In our research program for investigating anti-dementia agents from natural plant resources, we screened extracts from 15 spice samples. Among the plant extracts tested, ethyl acetate extract prepared from rhizome of Zingiber zerumbet showed potent activity. Activity-guided purification led to determination of afzelin and its acetylated compound as the active principles. Afzelin showed mod…

Latif MA et al, Cytotoxicity, Proliferation & Migration Rate Assessments of Human Dermal Fibroblast Adult Cells using Zingiber zerumbet Extract, Sains Malaysia, 48(1):121-7, 2019-Abstract: Zingiber zerumbet is a plant that is traditionally consumed in many countries, including Malaysia due to its therapeutic properties. Previously it has been proven to inhibit migration & proliferation of cancer cells. However, its effects on normal cells are still unknown & would be the main focus of this study. The cytotoxicity along with proliferation & migration activities were evaluated against human d…

Manandar B et al, Zerumbone-incorporated liquid crystalline nanoparticles inhibit proliferation & migration of non-small-cell lung cancer in vitro, Resaerch-gate, 2023--Abstract: Lung cancer is the second most prevalent type of cancer and is responsible for the highest number of cancer-related deaths worldwide. Non-small-cell lung cancer (NSCLC) makes up the majority of lung cancer cases. Zerumbone (ZER) is natural compound commonly found in the roots of Zingiber zerumbet which has recently demonstrated anti-cancer activity in both in vitro and in vivo studies. Despite their medical benefit…

Somchit M et al, Anti inflammatory property of ethanol & water extracts of. Zingiber zerumbet. Indian J Pharmacol. 2003;35:181-2.-Abstract: The antiinflammatory activities of mefenamic acid (Group 8), used as a standard reference drug, was found to be statistically significant. Generally, for all concentrations of aqueous extracts & NSAID, the peak effects against PGE2 were at 0.5 to 1.0 h. The ethanolic extract of ZZ was devoid of any antiinflammatory effect with 25 to 100 mg/kg concentrations (Group 4, 5 & 6). There was no significant difference at any time points when compared to control…

Sid Ahmed BHM et al. Antisecretory, Gastroprotective, Antioxidant & Anti-helicobacter Pylori Activity of Zerumbone from Zingiber Zerumbet (L.) Smith. PLoS One. 10(3): 1-21. 2015-Abstract: The aim of the current study is to evaluate the gastroprotective effect of zerumbone, the main bioactive compound of Zingiber zerumbet rhizome, against ethanol-induced gastric ulcer model in rats. Materials & Methods : Rats were pre-treated with zerumbone & subsequently exposed to acute gastric ulcer induced by absolute ethanol administration. Following treatment, gastric juice acidity, ulcer index, mucus …

Sriphana U et al, Antimalarial Activity & Cytotoxicity of Zerumbone Derivatives. Science-Asia. 39(1): 95-9. 2013-

Sulaiman MR et al. Preliminary Analysis of the Antinociceptive Activity of Zerumbone. Fitoterapia. 80(4): 230-2. 2009-

Tian M et al, Comparison of Chemical Composition & Bioactivities of Essential Oils from Fresh & Dry Rhizomes of Zingiber zerumbet (L.) Smith. Biomed Res. Int. 1-9.2020-Abstract: The chemical constituents and the antioxidant, antimicrobial, & cytotoxic activities of fresh rhizome essential oil (FR-EO) & dry rhizome essential oil (DR-EO) of Zingiber zerumbet (L.) Smith obtained from Southwest China were compared. Zerumbone was the predominant component in both FR-EO & DR-EO (75.0% & 41.9%, respectively). FR-EO, DR-EO, and zerumbone were all demonstrated to have significant antimicrobial capac…

Sulaiman MR et al. Anti-inflammatory Effect of Zerumbone on Acute & Chronic Inflammation Models in Mice. Fitoterapia. 81(7): 855-858. Doi: 10.1016/j.fitote.2010.05.009. 2010-

Zakaria ZA et al. Preliminary Analysis of the Anti-inflammatory Activity of Essential Oils of Zingiber zerumbet. Biol. Res. Nurs. 13(4): 425-32. 2011-Abstract: The present study was carried out to evaluate the acute & chronic anti-inflammatory activities of the essential oil of the rhizomes of Zingiber zerumbet (Zingiberaceae) using the carrageenan-induced paw edema & cotton pellet-induced granuloma tests, respectively. The effect of the essential oil on inflammatory- & noninflammatory-mediated pain was also assessed using the formalin test. Essential oil of ZZ at doses of 30, 100, & 300 m…

Widyastiwi W et al, Anxiolytic Activity of Ethanolic Extract of Three Species of Indonesian Lempuyang (Zingiber zerumbet, Zingiber aromaticum, & Zingiber americans). Open Access Maced J Med Sci [Internet]. 2022;10(A):695-701-Abstract: Lempuyang, or widely known as wild ginger, has been used in Indonesia as traditional medicine for a long time. However, scientific research supporting its medicinal use is still very limited. AIM: This research was conducted to investigate the anxiolytic activity of three species of Indonesian Lempuyang (Zingiber zerumbet, Zingiber aromaticum, & Zingiber ameri…

Yob BY et al, Zingiber zerumbet (L.) Smith: A Review of Its Ethnomedicinal, Chemical, & Pharmacological Uses. Evidence-based Comp & Alter Med. 1-12. 2011--Abstract: Zingiber zerumbet Sm., locally known to the Malay as “Lempuyang,” is a perennial herb found in many tropical countries, including Malaysia. The rhizomes of ZZ, particularly, have been regularly used as food flavouring & appetizer in various Malays' cuisines while the rhizomes extracts have been used in Malay traditional medicine to treat various types of ailments (e.g., inflammatory- & pain-mediated diseases, worm infestation & …

Ahmad N et al, ZINGIBER ZERUMBET: PHARMACOLOGICAL VALUES OF ZERUMBONE & THE EXTRACTION TECHNOLOGY EVOLUTION. J Teknologi, 85(2), 21-30. 2023-Abstract: Over the past eight decades, numerous research has been conducted on the extraction of Zingiber zerumbet rhizome. The mini-review includes information on the pharmacological properties of Zerumbone extracted from Z. zerumbet rhizome & the extraction methods conducted over the previous 80 years. Zerumbone is recognized as having a proven pharmacological effect & is a significant medicinal component used to treat various ailments. The pharmacol…

Jyothilakshmi M et al, Anti-inflammatory efficacy of the rhizome of Zingiber zerumbet–an in vitro study using THP1 cell line. J Med Plants Stud. 2016; 4:103–6-Abstract: Zingiber zerumbet is a perennial herb belonging to the family Zingiberaceae. Anti-inflammatory effect of the methanolic extract of the rhizome of Zingiber zerumbet collected from the district of Kottayam, Kerala was studied using THP1 cell line. The cell line was induced with lipopolysaccharide & the inhibitory effect of the extract on the activity of cyclooxygenase, lipoxygenase, myeloperoxidase & nitric oxide synthase was …

Ghasemzadeh A et al. Optimization of Microwave-assisted Extraction of Zerumbone from Zingiber zerumbet L. rhizome & Evaluation of Antiproliferative Activity of Optimized Extracts. Chem. Cent. J. 11(1): 1-10. 2017-Abstract:  The extraction of bioactive compounds from herbal materials requires optimization in order to recover the highest active dose. Response surface methodology was used to optimize variables affecting the microwave extraction of zerumbone from Zingiber zerumbet using the Box–Behnken design. The influence of variables, such as ethanol concentration (X1), microwave power (X2),…

Rahman HS et al, Zerumbone Induces G2/M Cell Cycle Arrest & Apoptosis Via Mitochondrial Pathway in Jurkat Cell Line. Nat. Prod. Commun. 9(9): 1237-42. 2014-Abstract: This investigation determined the anticancer properties of zerumbone (ZER) on the human T-cell (Jurkat) line using the MTT assay, microscopic evaluations, flow cytometric analyses, & caspase activity estimations. The results showed that ZER is selectively cytotoxic to Jurkat cells in a dose & time-dependent manner with IC50 of 11.9 ± 0.2, 8.6 ± 0.5 & 5.4 ± 0.4 μg/mL at 24, 48 & 72 hours of treatment, respectively. ZER did not p…

Abd Rashid R et al, The Antiproliferative Effects of Zingiber zerumbet Extracts & Fractions on the Growth of Human Breast Carcinoma Cell Lines, Malaysian J. Pharm. Sci. 3(1): 45-52. 2005-Abstract: Zingiber zerumbet, from Zingiberaceae family, is known for its medicinal properties. From the ethnopharmacology & laboratory studies, extracts & active component of this plant, zerumbone, were reported to have the antiinflammatory, antioxidant, antimicrobial & antitumour properties. However, no studies have been done to verify the antiproliferative activity of this plant extracts on breast cancer …

Azelan NA et al, Optimisation of Essential Oil Yield & Zerumbone Content in Zingiber zerumbet Extract using Hydrodistillation Process. Chem. Eng. Trans. 63: 595-600. 2018-Abstract: The optimisation of zerumbone content in essential oil of Zingiber zerumbet was investigated using hydrodistillation extraction method. The extractions were carried out to identify the optimum condition of the processing parameters (solvent-to-solid loading ratio, particle size & time) on the essential oil & zerumbone recovery. The extraction variables (solvent-to-solid ratio, particle size & time), on the other …

Chang JC et al, Acute & 28-Day Subchronic Oral Toxicity of an Ethanol Extract of Zingiber zerumbet (L.) Smith in Rodents, Evidence-Based Comp &  Alter Med, 2012-Abstract:The objective of this study was to evaluate the acute & subacute toxicity (28 days) of the ethanol extract of Z. zerumbet rhizomes (EEZZ) via the oral route in Wistar rats of both sexes. In the acute toxicity study, Wistar rats were administered a single dose of 15 g kg−1 of body weight by gavage, & were monitored for 14 days. EEZZ did not produce any toxic signs or deaths; the 50% lethal dose must be higher than 15 g kg−1.…

Padalia RC et al. Zingiber Zerumbet (L.) Roscoe ex Sm. from Northern India: Potential Source of Zerumbone Rich Essential Oil for Antiproliferative & Antibacterial Applications. Ind. Crops Prod. 112: 749-54. 2018-Abstract: Zingiber zerumbet (L.) Roscoe ex Sm. (Zingiberaceae), commonly known as bitter ginger, has long been used in traditional herbal medicine for the treatments of inflammations, rheumatism, sprains, colic pain, diarrhea, tonsillitis & various other ailments. The aim of the present study was to assess the chemical composition of the rhizome essential oil of ZZ grown in the foot…

Rawat A et al, Chemical composition of the essential oil from different plant parts of Zingiber zerumbet Sm. grown in the foothills of Uttarakhand, Biochem System & Ecol 108:104627, 2023-Abstract: Zingiber zerumbet (L.) (Family: Zingiberaceae) traditionally known as bitter ginger or shampoo ginger in India is cultivated mostly in tropical and subtropical regions all across the world. All the plant parts of ZZ. have wide applications for traditional as well as medicinal purposes. The present communication phytochemically analyzes different plant parts of ZZ viz.leaves (ZZLEO), cone (ZZCEO), …

Ramzan M et al, Assessment of the Phytochemical Analysis and Antimicrobial Potentials of Zingiber zerumbet, Molecules, 28(1):409, 2023-Abstract: The purpose of this study was to evaluate the component of Zingiber zerumbet extract in different solvents using high-performance liquid chromatography (HPLC), in addition to evaluate the antibacterial activity of these extracts, in combination with their antibiotic potential against four multi-drug resistant (MDR) bacterial strains (Lactobacillus acidophilus, Streptococcus mutans, Enterococcus faecalis & Staphylococcus aureus). Ethanol & the aqueo…

Ubol P et al, Repellency of essential oils extracted from Thai native plants against Aedes aegypti (Linn.) & Culex quinquefasciatus (Say). Pub Med, 2014-Abstract: Repellent activity of essential oils derived from 10 Thai native plants, belonging to three families were evaluated against female Aedes aegypti & Culex quinquefasciatus & to compare them with a commercial chemical repellent (DEET; N, N-diethyl-3-methylbenzamide 20% w/w; Sketolene Shield). Each test repellent was applied at 1, 5, & 10% concentrations for test by arm in cage method. The results showed significant differences in rep…

Rana A et al, Zingiber zerumbet (L)-Roscoe ex Smith: A potential source of Zerumbone rich essential oil, Int J of Phytomed & Related Ind, 14(1):162-8, 2022-Abstract: In the present study, essential oil isolated from Z. zerumbet rhizomes grown in Eastern India was analyzed by GC-FID & GC-MS. A total of seventeen aromatic compounds were identified representing 99.96% of the essential oil composition. The main oil constituents were zerumbone (70.60%), α-humulene (5.65%), humulene epoxide I (5.22%) & humulene epoxide II (5.71%), camphor (1.90%), camphene (3.47%), & cayophyllene oxide (2.52%). …

Santosh Kumar SC et al, Antibacterial & Antimutagenic Activities of Novel Zerumbone Analogues. Food Chem., 141: 1097-103, 2013-

Abdul ABH et al. Anticancer Activity of Natural Compound (Zerumbone) Extracted from Zingiber zerumbet in Human HeLa Cervical Cancer Cells. Int. J. Pharmacol. 4(3): 160-8. 2008-Abstract: A natural compound, Zerumbone was extracted, isolated & purified from the rhizomes of edible plant Zingiber zerumbet using methanol extraction & Column Chromatography (CC) method. The isolated & purified Zerumbone crystals were subjected to High Performance Liquid Chromatography (HPLC), Liquid Chromatography Mass Spectrometry (LCMS) & 13C NMR & 1H NMR analysis to confirm the purity, molecular weight and mole…

Hong TY et al, The Ethanol Extract of Zingiber zerumbet Rhizomes Mitigates Vascular Lesions in the Diabetic Retina. Vascul. Pharmacol. 76: 18-27. 2016-Abstract: diabetic retinopathy (DR) is a common diabetic eye disease which is well-known as the result of microvascular retinal changes. Although the ethanol extract from Zingiber zerumbet(L.) Smith rhizome (EEZZR) has been indicated to ameliorate hyperglycemia in diabetes, its protective effect on DR remains unclear. The aim of this study was to determine the effects of EEZZR on DR in streptozotocin (STZ) diabetic rats. Diabetic rats were tr…

Salleh MH et al, the protective effects of Zingiber zerumbet rhizome against pyrexia in rats Resaerch-gate, 2023-Abstract: The Zingiber zerumbet rhizomes are traditionally used to treat fever, & in vitro inhibitory effect of ethyl acetate extract from Zingiber zerumbet rhizomes (ZZEA) against DENV2 NS2B/NS3 has been reported early. This study was carried out to establish an acute toxicity profile & evaluate the anti-pyretic activities of ZZEA in yeast-induced pyrexia in rats. Methods The toxicity of ZZEA was evaluated using OECD Test Guideline 425 (tg-425) with minor modifications at concen…

Ungsurungsie M et al, Mutagenicity Screening of Popular Thai Spices. Food Chem. Toxicol. 20(5): 527-30. 1982-

Somchit MN et al. Anti-pyretic & Analgesic Activity of Zingiber zerumbet. Int. J. Pharmacol. 1(3): 277-80. 2005-Abstract: The objective of the present study was to evaluate the anti-pyretic & analgesic activities of aqueous & ethanol extracts of Zingiber zerumbet rhizomes. The anti-pyretic activity of ZZ (25, 50 & 100 mg kg<SUP>-1</SUP>) was studied in Brewer's yeast-induced pyrexia in rats. The analgesic activity of ZZ (10, 25, 50 & 100 mg kg<SUP>-1</SUP>) was studied using acetic acid-induced writhing in mice. Both aqueous & ethanol extracts of Zingiber zerumbet showed significant anti-py…

Tan JW et al, Major Bioactive Compounds in Essential Oils Extracted from the Rhizomes of Zingiber zerumbet (L) Smith: A Mini-Review on the Anti-allergic & Immunomodulatory Properties. Front. Pharmacol. 9: 1-8. 2018-Abstract: Zingiber zerumbet (L) Smith is part of the Zingiberaceae family, one of the largest families of the plant kingdom. ZZ is a perennial, aromatic and tuberose plant that grows in humid locations where its center of distribution is located in the South-East Asia region. This plant has been traditionally used in foods & beverages and for ornamental purposes. Although many st…

Taha NME et al, Potential Chemoprevention of Diethylnitrosamine-initiated & 2-acetylaminofluorene-promoted Hepatocarcinogenesis by Zerumbone from the Rhizomes of the Subtropical Ginger (Zingiber zerumbet). Chem. Biol. Interact. 186(3): 295-305. 2010-Abstract: Zerumbone, a monosesquiterpene found in the subtropical (Zingiber zerumbet, possesses antiproliferative properties to several cancer cells lines, including the cervical, skin & colon cancers. In this study, the antitumorigenic effects of ZER were assessed in rats induced to develop liver cancer with a single intraperitoneal injection o…

Albaayit SFA et al, Enzyme Inhibitory Properties of Zerumbone. Pakistan J. Agric. Sci. 58(3): 1207-9. 2021-

Amir SNKM et al, Modeling & Optimization of Pilot-scale Subcritical Water Extraction on Zingiber zerumbet by Central Composite Design. IOP Conf. Ser. Mater. Sci. Eng. 778(1): 2020-

Amir SNKM et al, Evaluation of Parameters for Subcritical Water Extraction of Zingiber Zerumbet Using Fractional Factorial Design. J. Teknol. 83(2): 143-50. 2021-Abstract: Zingiber zerumbet is recognized for decades for its usability as spice & condiment in food flavoring as well as having high medicinal properties. Up to date, there are limited literature on evaluation of the effects of multiple variables in details especially in pilot-scale subcritical water extraction (SWE) of ZZ. The aim for this study is to implement the fractional factorial design with five variables which are tempera…

Damodaran NP et al, Stereochemistry of Zerumbone. Tetrahedron Lett. 24: 1977-81. 1965-

Foong JN et al. Zerumbone-Loaded Nanostructured Lipid Carrier Induces Apoptosis of Canine Mammary Adenocarcinoma Cells. Biomed Res. Int. 1-18. Doi: 10.1155/2018/8691569. 2018-

Ahmad MS, Chemical study of Zerumbone from Zingiber Zerumbet, Smith. Master Thesis, UKM, 2006-

Damodaran N.P et al, Studies in sesquiterpenes-XXXVII. Sesquiterpenoids from the essential oil of Zingiber zerumbet smith. Tetrahedron, 24: 4113-22, 1968-Abstract: A complete analysis of the essential oil from the rhizomes of Zingiber zerumbet Smith, is given and the isolation of several new humulene-based sesquiterpenoids is described

Ibrahim MY et al, Evaluation of acute toxicity & the effect of single injected doses of zerumbone on the

Kalantari K et al. A Review of the Biomedical Applications of Zerumbone and the Techniques for Its Extraction from Ginger Rhizomes. Molecules. 22(10): 1-24. 2017-Abstract: Zerumbone (ZER) is a phytochemical isolated from the subtropical Zingiberaceae family & as a natural compound it has different biomedical properties such as antioxidant, anti-inflammatory anti-proliferative activity. ZER also has effects on angiogenesis & acts as an antitumor drug in the treatment of cancer, showing selective toxicity toward various cancer cell lines. Several techniques also have been established for extr…

Koga AY et al, Several aspects of Zingiber zerumbet: a review, Rev Bras de Farmacogn, 26(3):385-91, 2016-Abstract:Zingiber zerumbet is a perennial, aromatic & tuberose plant that grows in humid locations. Also known as bitter ginger, ZZ is traditionally found throughout Asia, where it is widely used in foods, beverages & for ornamental purposes. The viscous juice present in the inflorescence of the plant is rich in surfactants & is also known as “ginger shampoo”. The rhizome can be macerated in ethanol & used as a tonic & a stimulant. In Brazil ZZ is found in the Amazon region, in rural are…

Murakami A et al, Suppression of dextran sodium sulfate-induced colitis in mice by zerumbone, a subtropical ginger sesquiterpene, & Nimusolide: separately & in combination, Phytother Res, 31(4):924-38, 2019-Abstract:Ulcerative colitis (UC) & Crohn’s disease are inflammatory disorders of unknown cause & difficult to treat, though some synthetic chemicals, including ligands for peroxisome proliferator-activated receptors (PPARs), are anticipated to be useful drugs. In contrast, few food phytochemicals have been reported to suppress colitis in animal models. The present study was undertaken to…

Chang CJ et al, Absence of Genotoxic & Mutagenic Effects of Zingiber zerumbet (L.) Smith (Zingiberaceae) Extract. Evidence-based Comp Alter Med. 2012-

Chaudhuri A et al, An Overview on the Advances of Zingiber zerumbet, UK J. Pharm. Biosci. 6(5): 22. Doi: 10.20510/ukjpb/6/i5/177346. 2018-

Chaung HC et al, Anti-hypersensitive & anti-inflammatory activities of water extract of Zingiber zerumbet (L.) Smith, Food & Agric Immunol, 19:2, 2008-Abstract: Zingiber zerumbet Smith extract has been reported to have anti-inflammatory potential, especially in asthmatic patients. The aim of this study is to examine the capacity of water extract of ZZ (CC-ZWE) in protecting the lungs by inhibiting the release of inflammatory mediators during short-term treatment, & modulating cytokine gene expressions during long-term treatment. BALB/c mice were sensitised & challenged with ovalbumin to ind…

Ghazalee NS et al, Immunosuppressive Effects of the Standardized Extract of Zingiber zerumbet on Innate Immune Responses in Wistar Rats. Phytother. Res. 33(4): 929-38, 2019-Abstract: Zingiber zerumbet rhizome has been used in traditional medicine mainly for the treatment of various immune-inflammatory related ailments & has been shown to exhibit a wide spectrum of biological effects especially antioxidant & anti-inflammatory activities. The present study was aimed to investigate the immunosuppressive effects of the standardized 80% ethanol extract of ZZ at 100, 200, & 400 mg/kg on the innat…

Jafarian S et al, Effect of Zerumbone on scopolamine-induced memory impairment & anxiety-like behaviours in rats. Alzheimer’s Dement. 2019;(5):637-43-Abstract:

Kapitan O et al, in vitro antibacterial activity of the ethanolic extract of Puni (Zingiber zerumbet) from Timor Island. Savana Cendana, 2(02), 29-32. 2017-Abstract: Puni (Zingiber zerumbet) is a wildly grown plant that used by Timorese people to treat ulcerative lesions in Timor Island. This study aims to determine the antibacterial activity of plants extracts & know the content of active compounds on the most active extracts of plants. Extraction of active compounds was carried out using four solvents i.e., n-hexane, ethyl acetate, ethanol, & aqueous solvent. Antibacterial activity test w…

Dash B et al. Quantitative & Chemical Fingerprint Analysis for Quality Control of Zingiber zerumbet based on HPTLC Combined with Chemometric Methods. Plant Biosystem. An Int. J. Deal. with all Asp. Plant Biol. 155(4): 711-20. 2021-Abstract: A simple, reliable high-performance thin-layer chromatography (HPTLC) method was developed for chemical fingerprinting of Zingiber zerumbet & quantitative estimation of zerumbone. Thirty-six batches of ZZ were collected from five eco-regions of eastern India. Zerumbone content varied from 52.4 to 214.6 mg/g (dry weight) in methanolic extract of ZZ rhizom…

Dev S et al, Studies in Sesquiterpenes-XVI. Zerumbone, a Monocyclic Sesquiterpene Ketone. Tetrahedron. 8(3-4): 171-80. Doi: 10.1016/0040-4020(60)80027-0. 1960-

Hamid A et al. Cytotoxicity Evaluation of Zingiber Zerumbet Ethyl Acetate Extract on K-562, Erythroleukemia Cell Line. Pharmacogn. Mag. 14(57): 5430-5433. 2018-

Chhabra NP et al, Structure of zerumbone oxide: A new sesquiterpene epoxy ketone from Zingiber zerumbet Smith (wild ginger oil). Indian J Chem. 1975; 13: 222-4.

Koga AY et al, Several Aspects of Zingiber zerumbet: A Review. Rev. Bras. Farmacogn. 26(3): 385-91. Doi: 10.1016/j.bjp.2016.01.006. 2016-

Jantan I et al, Zerumbone from Zingiber Zerumbet Inhibits Innate & Adaptive Immune Responses in Balb/C mice. Int. Immunopharmacol. 73(June): 552-9. 2019-Abstract: Zerumbone exhibited various biological properties including in vitro immunosuppressive effects. However, its modulatory activity on the immune responses in experimental animal model is largely unknown. This investigation was conducted to explore the effects of daily treatment of zerumbone (25, 50, & 100 mg/kg) isolated from Zingiber zerumbet rhizomes for 14 days on various cellular & humoral immune responses in Balb/C mice. For me…

Malek SNA et al. Essential Oils of Zingiber ottensii Valet. & Zingiber zerumbet (L.) Sm. from Sabah,

Nik Norulaini NA et al. Optimization of SC-CO2 Extraction of Zerumbone from Zingiber zerumbet (L) Smith. Food Chem. 114(2): 702-5. Doi: 10.1016/j.foodchem.2008.09.075. 20&

Bandyopadhyay M et al, Antioxidant Activities & Cytotoxicity of Zingiber zerumbet (L.) Smith Rhizome, 102, J. Pharmacogn. Phytochem. 2(3): 102-8. 2013-

Dung NX et al, Chemical investigation of the aerial parts of Zingiber zerumbet (L.) Sm. from Vietnam. J Essent Oil Res. 1995; 7: 153-7.

Dev et al, Zerumbone: A Monocyclic Sesquiterpene Ketone. Chem. Ind. 1051. 1956-

Haque MA et al, recent Updates on the Phytochemistry, Pharmacological, and Toxicological Activities of Zingiber zerumbet (L.) Roscoe ex Sm. Curr. Pharm. Biotechnol. 18(9). 2018-

Khalid MH et al, Antinociceptive effect of the essential oil of Zingiber zerumbet in mice: Possible mechanisms, J of Ethnopharmacol, 137(1-:345-51, 2011-Abstract: The present study was conducted to investigate the possible mechanism of actions underlying the systemic antinociception activity of the essential oil of Zingiber zerumbet (EOZZ) in chemical-induced nociception tests in mice. Materials & methods: Acetic acid-induced abdominal constriction, capsaicin-, glutamate- & phorbol 12-myristate 13-acetate-induced paw licking tests in mice were employed in the study. In all experiments, EOZZ…

Haque MA et al, Recent updates on the phytochemistry, pharmacological, & toxicological activities of Zingiber zerumbet (L.) roscoe ex Sm. Curr Pharm Biotechnol. 2017;18(9):696–720-Abstract: Zingiber zerumbet (L.) Roscoe ex Sm. (family, Zingiberaceae) is a potent medicinal herb widely known as shampoo ginger & its rhizome is used in numerous ethnomedicinal applications including antipyretic, anti-inflammatory, antibacterial, anti-diarrheal, antidiabetics, carminative, & diuretic. The aim of this review was to bring together all the scientific updates on the phytochemistry & pharmacological a…

Sam MFR et al, A. Protective Effects of Zingiber Zerumbet Ethyl Acetate Extract on Hydrogen Peroxide-induced Damage of Red Blood Cells. Sains Malaysiana. 48(4): 781-90. 2019-Doi: 10.17576/jsm-2019-4804-10.

Chang CJ et al, The Ethanol Extract of Zingiber Zerumbet Smith Attenuates Non-alcoholic Fatty Liver Disease in Hamsters Fed on High-Fat Diet. Food Chem. Toxicol. 65(20101018): 33-42. 2014-Abstract: The beneficial effects of the ethanol extract of Zingiber zerumbet rhizome (EEZZR) for use in the treatment of non-alcoholic fatty liver disease (NAFLD) were investigated. Syrian golden hamsters were fed a high-fat diet to induce NAFLD. EEZZR (100, 200, or 300mg/kg) were orally administered by gavage once daily for 8weeks. The higher plasma levels of total cholesterol, triglycerides, free fatty a…

Duve RN et al. Highlights of the chemistry & pharmacology of wild ginger (Zingiber zerumbet Smith). Fiji Agric J. 1980; 42: 41-43.

Hasham R et al, A Comparative Study on Yield of Extract, Chemical Marker Content & Antioxidant Activity of Zingiber zerumbet & Zingiber Officinale. Conf. 2nd Int. Mater. Ind. Manuf. Eng. Conf. 5: 5-9. 2015-

Jyothilakshmi M et al, Antidermatophytic & Protease-inhibiting Activities of Zerumbone: A Natural Sesquiterpene from the Rhizome of Zingiber zerumbet (L.) Roscoe ex J.E; Smith. Pharmacogn. Mag. 13(49): 2-6. 2017-

Matsumura S et al, Search for β-Secretase Inhibitors from Natural Spices. Nat. Prod. Commun. 11(4): 507-10. 2016-

Jien TM et al, Zerumbone Induces Cytotoxicity & Inhibits Cell Migration of Human Colon Cancer Cells. Sains Malaysiana. 49(6): 1359-70. 2020-

Haque MA et al, Standardized Extract of Zingiber zerumbet suppresses LPS-induced pro-inflammatory responses through NF-κB, MAPK & PI3K-Akt signaling pathways in U937 macrophages. 54. Elsevier GmbH. 2019-

Mascolo N et al, Ethnopharmacologic investigation of. ginger (Zingiber officinale). Ethnopharmacology 1989; 27: 129-40

Dai JR et al, Zerumbone, an HIV-Inhibitory & Cytotoxic Sesquiterpene of Zingiber Aromaticum & Z. Zerumbet. Nat. Prod. Lett. 10(2): 115-8. 1997-Abstract: Zerumbone & 3“,4”-O-diacetylafzelin were isolated from organic extracts of rhizomes of Zingiber aromaticum (Zingiberaceae), & zerumbone & 4-O-acetylafzelin were obtained from organic extracts of entire plants of Z. zerumbet. Zerumbone exhibited HIV-inhibitory & cytotoxic activities, while the afzelins were inactive in both assays.

Faridah Q et al, Efficient in vitro regeneration of Zingiber zerumbet Smith (a valuable medicinal plant) plantlets from rhizome bud explants. Afr J of Biotechnol. 2011; 10(46):9303–8.

Kader G et al, Antimicrobial Activities of the Rhizome Extract of Zingiber zerumbet Linn. Asian Pac. J. Trop. Biomed. 1(5): 409-12. 2011-

Mohamad NE et al. Nanostructured Lipid Carrier Improved In vivo Anti-tumor & immunomodulatory Effect of Zerumbone in 4T1 Challenged Mice. RSC Adv. 5(28): 22066-74. 2015-

Rahman HS et al. Zerumbone-loaded Nanostructured Lipid Carriers: Preparation, Characterization, & Antileukemic Effect. Int. J. Nanomedicine. 8: 2769-81. Doi: 10.2147/IJN.S45313. 2013-

Lechat-Vahirua F et al, Aromatic Plants of French Polynesia. I. Constituents of the Essential Oils of Rhizomes of Three Zingiberaceae: Zingiber zerumbet Smith, Hedychium coronarium Koenig & Etlingera cevuga Smith. J. Essent. Oil Res. 5(1): 55-59. Doi: 10.1080/10412905.1993.9698170. 1993-

Nag A et al, Antioxidant activities & cytotoxicity of Zingiber zerumbet (L.) smith rhizome. J Pharmacogn &Phytochem. 2013; 2:102–8-Abstract: Zingiber zerumbet (L.) Smith, popularly known as shampoo ginger, is one of the most commonly used ingredients in Indo-Malaysian traditional medicines. The antioxidant & cytotoxic activities of the ethanolic extract of ZZ rhizome (ZZ) was investigated. 2,2-di(4-tert-octylphenyl)-1-picryl-hydrazyl (DPPH) & hydroxyl radical scavenging assays showed significant radical scavenging activities of ZZ. The extract was rich in polyphenol & flavonoids. Cytotoxici…

Raj S et al, Anti-microbial & Cytotoxic Activity of ZZ AMP, a Serine Protease Inhibitor (SPI) with Nutraceutical Potential from Rhizomes of Medicinal Plant, Zingiber Zerumbet. Nat. Prod. Res. 1-6. Doi: 10.1080/14786419.2022.2032046. `

Rahman HS et al. Biomedical Properties of Natural Dietary Plant Metabolite, Zerumbone, in Cancer Therapy & Chemoprevention Trials. Biomed Res. Int. 1-20. Doi: 10.1155/2014/920742. 2014-

Raina P et al, Zingiber zerumbet (L.) Roscoe ex Smith: A Potential Source of Zerumbone Rich Essential Oil. Med. Plants. 14(1): 162-8. 2022-

Shakaowat H et al, cytotoxicity of the rhizomes of medicinal plants, Pub-Med PMID;23569881, 2012-Abstract: To investigate the cytotoxicity of the crude ethanol extract of the rhizome of Zingiber zerumbet (Z. zerumbet) (L) Smith. & Curcuma zedoaria (C. zedoaria) Rosc. against Artemia salina Leach. Methods: Fresh rhizomes of ZZ & CZ were extracted separately in cold with ethanol (2.5 L) & after concentration a brownish syrupy suspension of ethanol extracts of ZZ & CZ was obtained. The cytotoxic effect of the crude ethanol extracts of both plants was determined by brine shrimp lethality bioass…

Rana VS et al, Essential Oil Composition, Antifungal, & Seedling Growth Inhibitory Effects of Zerumbone from Zingiber zerumbet Smith. J. Essent. Oil Res. 29(4): 320-9, 2017-

Sakinah SA et al, Zerumbone induced apoptosis in liver cancer cells via modulation of Bax/Bcl-2 ratio, Cancer cell inter, 7:4, 2007-Abstract: Zerumbone is a cytotoxic component isolated from Zingiber zerumbet Smith, a herbal plant which is also known as Lempuyang. This new anticancer bioactive compound from Z. zerumbet was investigated for its activity and mechanism in human liver cancer cell lines. Results: Zerumbone significantly showed an antiproliferative activity upon HepG2 cells with an IC50 of 3.45 ± 0.026 μg/ml. Zerumbone was also found to inhibit the proliferation of non-malignant …

Sharkawy NA et al, Curative Potential of Zingiber zerumbet on Paracetamol-induced Hepatotoxicity. J. Malaysian Soc. Appl. Biol. 48(3): 11-17, 2019-Abstract: Zingiber zerumbet is a type of wild ginger known worldwide for its medicinal values. Its constituents found in the rhizome suggest potent antioxidant & anti-inflammatory activities. As such, this study investigated the possible curative effects of ZZ rhizome ethanolic extract (ZZ) on Paracetamol (PCM)-induced hepatotoxicity. The plant was obtained from Sendayan, Negeri Sembilan and had been identified by the Biodiversity Unit of Univ Pu…

Rohmah J et al, ANTIOXIDANT ACTIVITIES USING DPPH, FIC, FRAP, & ABTS METHODS FROM ETHANOLIC EXTRACT OF LEMPUYANG GAJAH RHIZOME (Zingiber zerumbet (L.) Roscoe ex Sm. J K Riset, 7(2):152-66, 2022-Abstract: tn its use as traditional medicine, it is more often used than other types of lempuyang, namely the Lempuyang Gajah (Zingiber zerumbet (L.) Roscoe ex Sm.). Therefore, this study focused on the ZZ. Most of the biological activities reported for this plant are attributed to phenolic contents & volatile principles. Hence, a detailed investigation of antioxidant activity, flavonoid, & phenolic …